Composite

Part:BBa_K3425052

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)


Qβ5' recognition sequence of Qβ replicase with RBS

BBa_K3425052 is a construct containing the Qβ5' recognition sequence of Qβ replicase, BBa_K3425038, fused with the RBS designed for Type IIS iGEM Standard assembly, BBa_K3425032.

QB recognition site
References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/bacteriophage
//chassis/prokaryote/ecoli
//classic/rna/uncategorized
//direction/forward
Parameters
chassisE. coli
device_typeRNA functional element
directionforward
functionprotein expression regulation
rbsconstitutive